Excessive fluoride induces ovarian function impairment by regulating levels of ferroptosis in fluorosis women and ovarian granulosa cells.

The aim of this study was to investigate the role of ferroptosis in fluorosis women and the in vitro molecular mechanisms leading to ovarian dysfunction and abnormal hormone secretion by sodium fluoride (NaF) treatment of KGN cells. Fifty women with fluorosis as Fluorosis group and fifty healthy women as Control group were included in this study. The levels of lipid peroxidation and activities of antioxidant enzyme were assessed by photometric methods. The content of iron and glutathione (GSH) in serum was measured by microplate method. KGN cells were treated by different concentration of NaF (0, 1, 2, 4 and 8 ×10-3 M) for 24 h. The mRNA and protein expression levels of ferroptosis-related molecules, including glutathione peroxidase 4 (GPX4), solute carrier family 7 member (SLC7A11), nuclear factor erythroid 2-related factor 2 (Nrf2), ferritin heavy chain 1 (FTH1) and p53, were assessed by qRT-PCR and western blot analysis. Fluorosis group women had a significant higher levels of iron, Malondialdehyde (MDA), FSH and LH, and a lower levels of E2 and antioxidant enzyme in serum than that in the control group. The representative molecular changes of ferroptosis, such as the decrease in GPX4, Nrf2 and SLC7A11 expression (mRNA and protein expression), the increase in protein expression of p53, and a reduced level of E2 were observed in KGN cells treated by excessive NaF.It is concluded therefore that NaF increases the expression of p53 and inhibits ovarian granulosa cell ferroptosis preventive protein expression, resulting in abnormal hormone secretion and the ovarian dysfunction.

Analysis on the therapeutic effect of Cangfu Daotan Decoction combined with drospirenone and ethinylestradiol tablets (II) on patients with polycystic ovary syndrome.

OBJECTIVE: This study was designed to investigate the therapeutic effect of Cangfu Daotan Decoction (CDD) combined with drospirenone and ethinylestradiol tablets (II) on patients with polycystic ovary syndrome (PCOS). METHODS: Patients with PCOS were gathered from September 2020 to September 2022 and divided into the experimental group (n = 36), treated with CDD combined with drospirenone and ethinylestradiol tablets (II), and the control group (n = 41), received only drospirenone and ethinylestradiol tablets (II). Levels of sex hormone, obesity, blood glucose, blood lipid were detected and compared between the two groups pre- and post-treatment. The treatment efficacy, Traditional Chinese Medicine (TCM) syndrome score, adverse drug reactions, and pregnancy rate were compared as well. RESULTS: After treatment, the experimental group had a higher treatment efficacy (94.44% vs 73.17%, P < 0.05) and a higher pregnancy rate (44.44% vs 21.95%, P < 0.05) than the control group, but the difference in the incidence of adverse drug reactions was not statistically significant (P > 0.05). Compared with control group, TCM syndrome score and levels of fasting blood glucose, fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR), and waist circumference of the experimental group after treatment displayed remarkable reduction (P < 0.05), while the levels of estradiol (E2) and high-density lipoprotein cholesterol (HDL-C) showed a remarkable increase (P < 0.05). CONCLUSION: CDD in combination with drospirenone and ethinylestradiol tablets (II) may be effective in treating PCOS by improving obesity, glucose metabolism and lipid metabolism with no serious adverse events, making it a feasible clinical practice option.

Bubble Engineering on Micro-/Nanostructured Electrodes for Water Splitting.

Bubble behaviors play crucial roles in mass transfer and energy efficiency in gas evolution reactions. Combining multiscale structures and surface chemical compositions, micro-/nanostructured electrodes have drawn increasing attention. With the aim to identify the exciting opportunities and rationalize the electrode designs, in this review, we present our current comprehension of bubble engineering on micro-/nanostructured electrodes, focusing on water splitting. We first provide a brief introduction of gas wettability on micro-/nanostructured electrodes. Then we discuss the advantages of micro-/nanostructured electrodes for mass transfer (detailing the lowered overpotential, promoted supply of electrolyte, and faster bubble growth kinetics), localized electric field intensity, and electrode stability. Following that, we outline strategies for promoting bubble detachment and directional transportation. Finally, we offer our perspectives on this emerging field for future research directions.

Burden of female infertility in China from 1990 to 2019: a temporal trend analysis and forecasting, and comparison with the global level.

BACKGROUND: Infertility is a common reproductive disease that affects not only individuals and families, but also the growth of the social population. Hence, understanding the burden of female infertility in China and worldwide is of great significance for the development of infertility prevention and treatment strategies. METHODS: The Global Burden of Disease Study (GBD 2019) Data Resources were used to collect and collate relevant data on female infertility in China and worldwide from 1990 to 2019. The difference in the number, age-standardised prevalence rate (ASPR), disability-adjusted life years and age-standardised disability-adjusted life years rate (ASDR) of women with infertility in different periods and geographical areas were analysed. The autoregressive integrated moving average method was used to predict the ASPR and ASDR of female infertility in China and worldwide in the next 11years. RESULTS: In the past 30years, the number of female infertility cases increased by 7.06million in China and 56.71million worldwide. The corresponding average annual increase of ASPR was 10.10% and 7.28%, respectively, and that of ASDR was 0.08% and 0.79%, respectively. In addition, there are differences in age and time between Chinese and global female infertility. In 1990, the crude prevalence rate of female infertility was the highest in women aged 40-44years and 35-39years in China and worldwide, respectively. In 2019, the crude prevalence rate of female infertility was still the highest in women aged 40-44years in China, whereas that around the world reached the highest in women aged 30-34years, which was significantly earlier. The forecast for the next 11years suggests that the ASPR and ASDR for female infertility in China will first rise and then decline, but the overall magnitude of change is not very significant, whereas the ASPR and ASDR for female infertility globally are still on the rise. The ASPR value of female infertility is expected to be 5025.56 in 100 000 persons in China and 3725.51 in 100 000 persons worldwide by 2030. The ASDR value of female infertility is expected to be 26.16 in 100 000 persons in China and 19.96 in 100 000 persons worldwide by 2030. CONCLUSION: The burden of female infertility is still increasing in China and worldwide. Therefore, it is of great significance to pay more attention to infertile women, and advocate a healthy lifestyle to reduce the burden of disease for infertile women.

Theoretical optimization of a frequency-domain- based color polarization interference sensor via coupling with a Gaussian beam.

In this paper, a Gaussian-beam-coupled color polarization interference (CCPI) sensor with an adjustable digital Gaussian filter was proposed theoretically in the frequency domain. The coupling principle of a traditional color polarization interference (CPI) sensor and a Gaussian beam was investigated, and sensitivity optimization was then implemented. The formation of a doublet is related to the shape of a CPI curve, expected wavelength, and FWHM of the Gaussian beam. Based on the calculated results, the angle between the two polarizers and the obliquity of the wave plate are the two most significant adjusting parameters. The sensitivity of a CCPI sensor is approximately twofold higher than that of a traditional CPI sensor. The sensing range is also related to the FWHM of the Gaussian beam and a larger FWHM usually means a wider sensing range. We also found that the sensitivity would reach a maximum when the incident angle is near the total internal reflection angle. Besides, a longer incident wavelength usually corresponds to a higher sensitivity and the sensitivity could reach as high as 9270 nm/refractive index unit when the resonance wavelength is 977.40 nm.

Dislocation Mechanism and Grain Refinement of Surface Modification of NV E690 Cladding Layer Induced by Laser Shock Peening.

To investigate the relationship between the dislocation configuration and the grain refinement in the NV E690 cladding layer caused by laser shock peening, NV E690 high-strength steel powder was used to repair prefabricated pits in samples of 690 high-strength steel by laser cladding, where the laser shock peening of the cladding layer was performed by laser shock at different power densities. X-ray diffraction, scanning electron microscopy, and transmission electron microscopy were used to observe the microstructures of these samples before and after the laser shock process. The results showed that the metallurgical bonding between the cladding layer and the substrate after laser cladding repair was good. When the laser power density was 4.77 GW/cm2, multiple edge dislocations, dislocation dipoles, and extended dislocations were distributed over the cladding layer. When the laser power density was 7.96 GW/cm2, a geometrically necessary dislocation divided the large original grain into two subgrains with different orientations. When the laser power density was 11.15 GW/cm2, geometric dislocations divided the entire large grain into fine grains. The grain refinement model of the NV E690 cladding layer, when treated by laser shock peening, can describe the grain refinement process induced by the dislocation movement of this cladding layer.

Downregulation of a mitochondrial micropeptide, MPM, promotes hepatoma metastasis by enhancing mitochondrial complex I activity.

We and others have shown that MPM (micropeptide in mitochondria) regulates myogenic differentiation and muscle development. However, the roles of MPM in cancer development remain unknown. Here we revealed that MPM was downregulated significantly in human hepatocellular carcinoma (HCC) tissues and its decrease was associated with increased metastasis potential and HCC recurrence. Gain- and loss-of-function investigations disclosed that in vitro migration/invasion and in vivo liver/lung metastasis of hepatoma cells were repressed by restoring MPM expression and increased by silencing MPM. Mechanism investigations revealed that MPM interacted with NDUFA7. Mitochondrial complex I activity was inhibited by overexpressing MPM and enhanced by siMPM, and this effect of siMPM was attenuated by knocking down NDUFA7. The NAD+/NADH ratio, which was regulated by complex I, was reduced by MPM but increased by siMPM. Treatment with the NAD+ precursor nicotinamide abrogated the inhibitory effect of MPM on hepatoma cell migration. Further investigations showed that miR-17-5p bound to MPM and inhibited MPM expression. miR-17-5p upregulation was associated with MPM downregulation in HCC tissues. These findings indicate that a decrease in MPM expression may promote hepatoma metastasis by increasing mitochondrial complex I activity and the NAD+/NADH ratio.

Assessment of hearing loss induced by tympanic membrane perforations under blast environment.

PURPOSES: This study provides an approach to estimating tympanic membrane perforation-induced hearing loss (HL) using a human middle ear model. METHODS: Sixty-one cases of tympanic membrane perforation originating from fireworks were reported from the Ear-Nose-Throat Department. The otoscope, audiometry data and diagnosis records were organized, and gender, age, etiology, perforation size and diseased ear side were classified as independent variables. A multinomial regression model was used to analyze the potential effects of the variables on HL. Meanwhile, a human middle ear model was implemented to calculate the ensued HL resulting from different perforation areas and sites. In addition, linear regression models were used to establish functions between perforation size and HL. RESULTS: The audiometry data indicate that HL at high frequencies (f > 2 kHz) is much more profound than that at the speech frequency band (f < 1 kHz). Compared with mild HL (<15 dB), mediate HL (15-30 dB) was correlated with the perforation area (p < 0.05, 95% CI), while severe HL (>30 dB) was affected by both perforation size and age (p < 0.05, 95% CI). However, other factors, including gender and diseased ear side, do not show a statistically significant effect on HL. Furthermore, the Kruskal-Wallis test result reveals that HL at frequencies of 0.25 kHz ≤ f ≤ 8 kHz is strongly associated with the perforation size (p < 0.05, 95% CI). CONCLUSIONS: It is conclusive that HL is positively proportional to the perforation size. However, HL is not correlated with the perforation site for small perforation areas of < 10% (p > 0.05, 95% CI).

A study on the effect of ligament and tendon detachment on human middle ear sound transfer using mathematic model.

The objective of this study is to investigate the effects of ligament and tendon detachment on human middle ear sound transfer. For this purpose, a geometric human middle ear model was reconstructed based on the computed tomography scanning data of the temporal bones from healthy adult volunteers. For the ear model, pars tensa was assumed to be fit for a 5-parameter Maxwell model and inverse method was used to obtain the necessary coefficients. Furthermore, frequency response method was implemented to investigate the vibration behaviors of tympanic membrane umbo and stapes footplate under an acoustic stimulus of 90 dB within 0.2-8 kHz. Meanwhile, nine patterns of fractured ligaments and tendons, whose effects on the middle ear sound transfer function were simulated by setting free the nodes of the ligaments and tendons of interest. The results indicate that the displacement of tympanic membrane umbo and stapes footplate as well as the velocity transfer function lies within the bounds of the published experimental data. The detachments of ligaments or tendons except for lateral mallear ligament may incur both gains as much as 15 dB and losses of -8 dB in the velocity of stapes footplate at low frequencies (f≤ 1 kHz), while no significant changes were observed at high frequencies (f > 1 kHz). However, detachment of the ligaments or tendons induces tiny changes in the displacement of stapes footplate at the frequencies of 0.2-8 kHz.

Risks of Ear Complaints of Passengers and Drivers While Trains Are Passing Through Tunnels at High Speed: A Numerical Simulation and Experimental Study.

Ear complaints induced by interior pressure transients are common experiences for passengers and crew members when high-speed trains are passing through tunnels. However, approaches to assessing the risks of the pressure-related aural discomfort have not been reported until recently. The objective of this study was to evaluate the hazards of interior pressure transients of high-speed train on human ears combining the effects of operation speed and seal index. Moving model tests were conducted to obtain the pressure transients when the model train runs in the tunnel. The recorded data were transformed into the interior pressures by empirical formula. Furthermore, the aural sensations were divided into four levels hierarchically and the range for each level was derived by logistic regression analysis method and represented by three biomechanical metrics. Furthermore, a human middle ear finite element (FE) model was used to simulate its dynamics under the interior pressures. The results indicate that lifting operation speed from 250 km/h to 350 km/h in tunnel will prolong the duration of ear complaints by more than two times whereas improving the seal index from 4 s to 12 s will reduce the incidences of the onset of tinnitus and hearing loss by more than ten times. In addition, the duration of aural comfort shortens from the head car to the tail car against the running direction. It is desirable that enhancing the seal index improve the aural sensations of the passengers and crew members considering the lifting operation speed of high-speed train.

Tunnel driving occupational environment and hearing loss in train drivers in China.

OBJECTIVE: Hearing loss caused by high levels of noise is a potential occupational health disorder among train drivers around the world. This study aims to investigate the relationship between tunnel driving occupational environment and hearing loss in train drivers, to provide some insights into helping reduce hearing loss among train drivers. METHODS: This study analysed cross-sectional data for 1214 train drivers who work at China Railway Guangzhou Group. Health examination was taken by physicians with professional licences, and audiometric testing was performed by health technicians in a sound-isolated room. T/R is defined as the ratio of the length of the tunnels to the length of the railway along drivers' work routes. Different multivariate models and stratified models were established for sensitivity analysis. A multivariable logistic regression model was used to estimate the ORs of hearing loss associated with tunnel driving occupational environment. RESULTS: The adjusted OR for high-frequency hearing loss in association with the highest T/R levels (30%-45%) versus the lowest T/R levels (<15%) was 3.72 (95% CI 1.43 to 9.69). The corresponding OR for speech-hearing loss was 1.75 (95% CI 0.38 to 8.06). The sensitivity analysis shows our results are suitable for various alternative models. CONCLUSIONS: This study found that there was a significant association between tunnel driving occupational environment and hearing loss. Train drivers who work in a higher T/R environment have worse hearing loss.

Green material selection for sustainability: A hybrid MCDM approach.

Green material selection is a crucial step for the material industry to comprehensively improve material properties and promote sustainable development. However, because of the subjectivity and conflicting evaluation criteria in its process, green material selection, as a multi-criteria decision making (MCDM) problem, has been a widespread concern to the relevant experts. Thus, this study proposes a hybrid MCDM approach that combines decision making and evaluation laboratory (DEMATEL), analytical network process (ANP), grey relational analysis (GRA) and technique for order performance by similarity to ideal solution (TOPSIS) to select the optimal green material for sustainability based on the product's needs. A nonlinear programming model with constraints was proposed to obtain the integrated closeness index. Subsequently, an empirical application of rubbish bins was used to illustrate the proposed method. In addition, a sensitivity analysis and a comparison with existing methods were employed to validate the accuracy and stability of the obtained final results. We found that this method provides a more accurate and effective decision support tool for alternative evaluation or strategy selection.